Primases are essential genes and play a fundamental role in genome
replication. Currently there is only limited knowledge on the detailed
molecular mechanisms underlying primase activity. In this project the
structure and dynamics of the reaction steps of a primase during
initiation (dinucleotide formation) and elongation shall be elucidated.
We will study the archaeoeukaryotic primase of the replication protein
of the plasmid pRN1 (EMBO 22, 2516; NSMB 11, 157; NAR 35, 5635; NAR 38,
6707) with advanced NMR methods, isothermal calorimetry and fluorescence
spectroscopy. Mutant proteins at selected residues will be constructed
and characterized to unravel the contribution of these residues to
individual steps in the reaction cycle.
Working place of the PhD student is the University of Applied Sciences
Northwestern Switzerland in Basle in the group of Prof. Dr. Georg Lipps.
This SNF funded project is a close cooperation with the group of Prof.
Dr. Frédéric Allain (ETH Zurich). A PostDoc is working in his group is
part of the funded project. Basle is located in the vivid trinational
region (Switzerland, Germany, and France) in a beautiful landscape.
The applicant must have an MSc degree in biology, chemistry or related
disciplines. Please send your application (CV, copies of certificates,
overview of your studies, names and contact information of two
references) in a single pdf file to Prof. Dr. Georg Lipps by July 31th.
Please include pdf-files of your master thesis and bachelor thesis as
well. Looking forward to your application!
Methoden:
We apply numerous techniques and experimental approaches: molecular
biology, protein purification, enzyme assays, HPLC, ITC, NMR and
fluorescence spectroscopy. Hands-on experiences with some of these
methods are a plus.
Anfangsdatum: 1. September 2012
geschätzte Dauer: 3 years
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